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Duclos, Guillaume; Adkins, Raymond; Banerjee, Debarghya; Peterson, Matthew S. E.; Varghese, Minu; Kolvin, Itamar; Baskaran, Arvind; Pelcovits, Robert A.; Powers, Thomas R.; Baskaran, Aparna; et al (, Science)Topological structures are effective descriptors of the nonequilibrium dynamics of diverse many-body systems. For example, motile, point-like topological defects capture the salient features of two-dimensional active liquid crystals composed of energy-consuming anisotropic units. We dispersed force-generating microtubule bundles in a passive colloidal liquid crystal to form a three-dimensional active nematic. Light-sheet microscopy revealed the temporal evolution of the millimeter-scale structure of these active nematics with single-bundle resolution. The primary topological excitations are extended, charge-neutral disclination loops that undergo complex dynamics and recombination events. Our work suggests a framework for analyzing the nonequilibrium dynamics of bulk anisotropic systems as diverse as driven complex fluids, active metamaterials, biological tissues, and collections of robots or organisms.more » « less
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